CIRCINATUM-ingeleraz

JULKAISUJA - PUBLICATIONS
01/2005
EMERGING FUSARIUM-MYCOTOXINS IN FINLAND
Marika Jestoi

University of Turku,
Department of Biochemistry and Food Chemistry

National Veterinary and Food Research Institute
Department of Chemistry

EMERGING FUSARIUM-MYCOTOXINS IN FINLAND
Marika Jestoi

ACADEMIC DISSERTATION
National Veterinary and Food Research Institute (EELA), Department of Chemistry
and
Department of Biochemistry and Food Chemistry, University of Turku

Supervised by: Professor Rainer Huopalahti
Department of Biochemistry and Food Chemistry
University of Turku, Finland
Dr. Aldo Rizzo
Department of Chemistry
National Veterinary and Food Research Institute (EELA), Finland


Reviewed by: Dr. Antonio Logrieco
Institute of Sciences of Food Production of the Italian National Research Council (ISPA)
Bari, Italy
Professor Risto Kostiainen
Department of Pharmacy
University of Helsinki, Finland


ISSN 1458-6878
ISBN 952-5568-02-4 (Print)
ISBN 952-5568-03-2 (PDF)
Helsinki 200551
Marika Jestoi, Academic Dissertation
Appendix 2.

The Fusarium-species reported to produce beauvericin.

Fusarium spp. Reference(s)
F. acuminatum Logrieco et al. 1998
F. acutatum Fotso et al. 2002
F. anthophilum Logrieco et al. 1998
F. avenaceum Logrieco et al. 1998, 2002, Morrison et al. 2002c
F. beomiforme Logrieco et al. 1998
F. circinatum Fotso et al. 2002
F. concentricum Fotso et al. 2002
F. dlamini Logrieco et al. 1998
F. equiseti Logrieco et al. 1998
F. fujikuoi Moretti et al. 1996
F. globosum Shephard et al. 1999
F. guttiforme Fotso et al. 2002
F. konzum Leslie et al. 2004
F. langsethiae Thrane et al. 2004
F. longipes Logrieco et al. 1998
F. nygamai Logrieco et al. 1998
F. oxysporum Logrieco et al. 1998
F. poae Logrieco et al. 1998, 2002, Thrane et al. 2004
F. proliferatum Moretti et al. 1994, 1996, Plattner & Nelson 1994, Bottalico et al. 1995,
Munkvold et al. 1998, Shephard et al. 1999, Abbas et al. 1999
F. pseudoanthophilum Fotso et al. 2002
F. sambucinum Logrieco et al. 1998
F. semitectum Gupta et al. 1991
F. sporotrichioides Thrane et al. 2004
F. subglutinans Gupta et al. 1991, Logrieco et al 1993a,b, 1998, Moretti et al. 1995,
Sewram et al. 1999a, Shephard et al. 1999, Torres et al. 2001
F. tricinctum Logrieco et al. 2002
F. verticillioides (F. moniliforme) Bottalico et al. 1995, Srobarova et al. 2002Appendix 5.
Different toxic actions reported for beauvericin in in vitro -tests.
Property Organism/tissue/cell line Observed effect(s)/special remarks Effective Reference
concentration/dose
ANTIMICROBIAL Gram(+) bacteria, fungi viability not reported Hamill et al. 1969
bacteria isolated from especially Gram(+)bacteria sensitive 0.1-25 μg Castlebury et al. 1999
mammalian intestinal tract
Mycobacterium tuberculosis, viability minimal inhibitory Nilanonta et al. 2002
Plasmodium falciparum concentration (MIC)
1.6 μg/ml (M.t),
IC50 1.3 μg/ml (P.f)
Salmonella typhimurium, BEA not mutagenic in Ames test; EC50 94 ± 9 μg/ml Fotso & Smith 2003
Vibrio fisheri moderately toxic in bioluminescence assay
INSECTICIDAL brine shrimp (Artemia salina), mortality not reported Hamill et al. 1969
mosquito larvae (Aedes aegypti)
blowfly (Calliphora erythrocephala), mortality: 15 %, 39-86 % 5 μg; 10-20 μg/ml Grove & Pople 1980
mosquito larvae (Aedes aegypti)
Colorado potato beetle mortality LC50 (50 % lethal Gupta et al. 1991
(Leptinotarsa decemlineata) concentration)
663 ppm ; LC90 1196 ppm
Culex pipiens autogenicus mortality (44 %) ; vacuolization, 0.1 mg/ml; Zizka & Weiser 1993
mitochondrial damage, ruptured LD50 >0.01 mg/ml
membranes of midgut epithelia
Mysidopsis bahia mortality LC50 (50 % lethal Genther et al. 1994
concentration) 0.56 mg/l
mosquito larvae (Aedes aegypti) mortality LC50 (50 % lethal Gupta et al. 1995
concentration) 26 ppm
parasites (Nippostrongylus mortality; ENNs more effective 100 μg/ml Pleiss et al. 1996
brasiliensis, Trichinella spiralis,
Heterakis spumosa)
CYTOTOXIC Leucophaea maderae viability; damage of 1-10 ppm Vey et al. 1973
(primary and cardiac cells) mitochondria and nucleus
human blood platelets aggregation, no increase in cytoplasmic 20 μM Massini & Näf, 1980
Ca2+-concentration
smooth muscle selective inhibition on the tonic IC50 2.8 x 10-7 M Nakajyo et al. 1987
(guinea pig taenia coli) component of the high K+-induced
contraction; intracellular Na+ or K+
was not affected ➔ inhibitory action
due to the inhibition of the entry of Ca2+
through the voltage-dependent Ca2+
-channel opened by depolarization and
following increase in cytoplasmic Ca2+
-concentration
P815, Yac-1, EL-4 increase of intracellular Ca2+, DNA- 0-50 μM Ojcius et al. 1991
(murine tumor cells) fragmentation ➔ apoptosis
J774 (mouse macrophages) specific inhibition of ACAT CD50 (50 % cell damaging Tomoda et al. 1992
concentration) 11 μM
55
Marika Jestoi, Academic Dissertation
Property Organism/tissue/cell line Observed effect(s)/special remarks Effective Reference
concentration/dose
CYTOTOXIC HeLa (human cervical carcinoma), decrease of metabolic activity CC50 4-25 μM Macchia et al. 1995
continued HEP G2 (human hepatoma),
IARC BL-41 (human Burkitt’s
lymphoma), U937 (human
hystiocytic lymphoma), RBL-1
(rat basophilic/mast cell leukemia),
CV-1 (monkey kidney nonneoplastic
fibroblast-like cells),
SF-9 (lepidopteran)
astrogial cells (rat brain cells) increase of intracellular Ca2+, 10 μM Holownia et al. 1997
DNA fragmentation, apoptosis
H-69 (human nonmalignant DNA fragmentation, apoptosis 0-25 μmol/l Harnois et al. 1997
cholangiocyte)
L-1210-Fas (mouse lymphocytes), infection with Toxoplasma gondii 10-20 μM Nash et al. 1998
A20 (mouse B cell lymphoma), protected cells from apoptosis
WEHI-231 (mouse pre-B induced by BEA
leukemia cell)
H69 (human cholangiocyte) apoptosis in 40 % of the cells; effect 5 μM Que et al. 1999
inhibited by glycine ursodeoxycholic
acid (GUDC)
smooth and cardiac muscle reduced the contraction of terminal ilea IC50 0.86 mM; Lemmens-Gruber
(terminal ilea and papillary concentration-dependently; the force IC50 18 μM et al. 2000
muscle cells of guinea pigs) of contradiction of papillary muscles
decreased; BEA blocks the Ca2+-current
and affects also the Na+- and K+-currents
of the cells
NG105-18 (mouse neuroblastoma block of L-type Ca2+-channels; IC50 4 μM Wu et al. 2002
and rat glioma hybrid cell line) dose-dependent
KB (human epidermoid decrease in metabolic activity IC50 10->20 μg/ml Nilanonta et al. 2002
carcinoma), BC-1 (human breast
cancer), Vero (monkey kidney
fibroblast)
SF-9 (lepidopteran) viability, dose- and time-dependent action CC50 85-2.5 μM (4-120 h) Caló et al. 2003
smooth muscle (ventricular increase of intracellular Ca2+ and Na+, 10 μM Kouri et al. 2003
myocytes of guinea pig) decrease of intracellular K+; BEA forms
ion channels into the cell membrane
PBMC (turkey peripheral DNA fragmentation, condensation of 8 μM Dombrink-Kurtzman
lymphocytes) chromatin apoptosis; additive effect with FB1 2003
BEC (mouse biliary epithelial cells) apoptosis; dose- and time-dependent action 5-50 μM Iwata et al. 2003
U-937 (human myeloid), decrease of metabolic activity CC50 15-30 μM Caló et al. 2004
HL-60 (human myeloid)
PK15 (porcine kidney cells) decrease in metabolic activity EC50 12 μM Uhlig et al. 2004b
SF-9 (S.frugiperda) decrease in metabolic activity IC50 2.5 μM; Fornelli et al. 2004
CC50 3.0 μM